| Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE) | |
Liu L(刘丽) ; Chen YY(陈艳艳); Meng YH(孟永宏); Chen S(陈涉); Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
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| Source Publication | THIN SOLID FILMS |
| 2011 | |
| Pages | 2758-2762 |
| Conference Name | 5th International Conference on Spectroscopic Ellipsometry |
| Conference Date | MAY 23-29, 2010 |
| Conference Place | Albany, NY |
| Abstract | The biosensor based on the total internal reflection imaging ellipsometry (TIRIE) is realized as an automatic analysis method for protein interaction processes in real-time, with high throughput and label-free. An evanescent wave is used as the optical probe to monitor bio-molecular interactions on a chip surface with a high sensitivity due to its phase sensitive property. In this paper, the technique is optimized with a polarization setting, a spectroscopic light source and a low noise CCD detector to improve the performance of the biosensor in sensitivity and detection limit, as evidenced by a quantitative detection of Hepatitis B virus surface antigen (HbsAg) with concentrations of 8, 16, 32, 64, 125 and 250 ng/ml. The sensitivity is increased by one order of magnitude and the detection limit has been extended more than 50 times for HbsAg detection. (C) 2011 Elsevier B.V. All rights reserved. |
| Keyword | Sensitivity Improvement Imaging Ellipsometry Total Internal Reflection Biosensor Hbsag Detection |
| Department | NML纳米生物光学 |
| Funding Organization | Univ Albany, Coll Nanoscale & Engn |
| URL | 查看原文 |
| Indexed By | CPCI |
| Language | 英语 |
| Document Type | 会议论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/45319 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Jin, G (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Liu L,Chen YY,Meng YH,et al. Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)[C]THIN SOLID FILMS,2011:2758-2762. |
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