| Surface-Bound Selectin-Ligand Binding Is Regulated By Carrier Diffusion | |
Sun GY(孙淦云) ; Zhang Y(章燕); Huo B(霍波); Long M(龙勉); Long M
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| Source Publication | European Biophysics Journal With Biophysics Letters
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| 2009 | |
| Pages | 701-711 |
| ISSN | 0175-7571 |
| Abstract | Two-dimensional (2D) kinetics of receptor-ligand interactions governs cell adhesion in many biological processes. While the dissociation kinetics of receptor-ligand bond is extensively investigated, the association kinetics has much less been quantified. Recently receptor-ligand interactions between two surfaces were investigated using a thermal fluctuation assay upon biomembrane force probe technique (Chen et al. in Biophys J 94:694-701, 2008). The regulating factors on association kinetics, however, are not well characterized. Here we developed an alternative thermal fluctuation assay using optical trap technique, which enables to visualize consecutive binding-unbinding transition and to quantify the impact of microbead diffusion on receptor-ligand binding. Three selectin constructs (sLs, sPs, and PLE) and their ligand P-selectin glycoprotein ligand 1 were used to conduct the measurements. It was indicated that bond formation was reduced by enhancing the diffusivity of selectin-coupled carrier, suggesting that carrier diffusion is crucial to determine receptor-ligand binding. It was also found that 2D forward rate predicted upon first-order kinetics was in the order of sPs > sLs > PLE and bond formation was history-dependent. These results further the understandings in regulating association kinetics of surface-bound receptor-ligand interactions. |
| Keyword | Association Kinetics Regulating Factors Carrier Diffusion Optical Trap Selectin Ligand Mediated Cell-adhesion P-selectin Glycoprotein Ligand-1 Optical Tweezers Shear-flow Human Neutrophils Force Microscopy Association Rate Contact Area Kinetics |
| DOI | 10.1007/s00249-009-0428-y |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000265917300014 |
| WOS Keyword | MEDIATED CELL-ADHESION ; P-SELECTIN ; GLYCOPROTEIN LIGAND-1 ; OPTICAL TWEEZERS ; SHEAR-FLOW ; HUMAN NEUTROPHILS ; FORCE MICROSCOPY ; ASSOCIATION RATE ; CONTACT AREA ; KINETICS |
| WOS Research Area | Biophysics |
| WOS Subject | Biophysics |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/26708 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Long M |
| Recommended Citation GB/T 7714 | Sun GY,Zhang Y,Huo B,et al. Surface-Bound Selectin-Ligand Binding Is Regulated By Carrier Diffusion[J]. European Biophysics Journal With Biophysics Letters,2009:701-711. |
| APA | 孙淦云,章燕,霍波,龙勉,&Long M.(2009).Surface-Bound Selectin-Ligand Binding Is Regulated By Carrier Diffusion.European Biophysics Journal With Biophysics Letters,701-711. |
| MLA | 孙淦云,et al."Surface-Bound Selectin-Ligand Binding Is Regulated By Carrier Diffusion".European Biophysics Journal With Biophysics Letters (2009):701-711. |
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