Knowledge Management System of Institue of Mechanics, CAS
| A Novel Counter Sheet-Flow Sandwich Cell Culture Device For Mammalian Cell Growth In Space | |
Sun SJ(孙树津) ; Gao YX(高宇欣); Shu NJ; Tang ZM(唐泽眉); Tao ZL(陶祖莱); Long M(龙勉); Sun, SJ (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China.
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| Source Publication | Microgravity Science And Technology
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| 2008 | |
| Pages | 115-120 |
| ISSN | 0938-0108 |
| Abstract | Cell culture and growth in space is crucial to understand the cellular responses under microgravity. The effects of microgravity were coupled with such environment restrictions as medium perfusion, in which the underlying mechanism has been poorly understood. In the present work, a customer-made counter sheet-flow sandwich cell culture device was developed upon a biomechanical concept from fish gill breathing. The sandwich culture unit consists of two side chambers where the medium flow is counter-directional, a central chamber where the cells are cultured, and two porous polycarbonate membranes between side and central chambers. Flow dynamics analysis revealed the symmetrical velocity profile and uniform low shear rate distribution of flowing medium inside the central culture chamber, which promotes sufficient mass transport and nutrient supply for mammalian cell growth. An on-orbit experiment performed on a recovery satellite was used to validate the availability of the device. |
| Keyword | Counter Sheet-flow Sandwich Cell Culture Mammalian Cell Growth Microgravity Dogfish Scyliorhinus Stellaris Shear-stress Spaceflight Microgravity Bioreactors Exchange Gills Blood Water Wall |
| DOI | 10.1007/s12217-008-9008-5 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000260252100009 |
| WOS Keyword | DOGFISH SCYLIORHINUS STELLARIS ; SHEAR-STRESS ; SPACEFLIGHT ; MICROGRAVITY ; BIOREACTORS ; EXCHANGE ; GILLS ; BLOOD ; WATER ; WALL |
| WOS Research Area | Engineering ; Thermodynamics ; Mechanics |
| WOS Subject | Engineering, Aerospace ; Thermodynamics ; Mechanics |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/25860 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Sun, SJ (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China. |
| Recommended Citation GB/T 7714 | Sun SJ,Gao YX,Shu NJ,et al. A Novel Counter Sheet-Flow Sandwich Cell Culture Device For Mammalian Cell Growth In Space[J]. Microgravity Science And Technology,2008:115-120. |
| APA | 孙树津.,高宇欣.,Shu NJ.,唐泽眉.,陶祖莱.,...&Sun, SJ .(2008).A Novel Counter Sheet-Flow Sandwich Cell Culture Device For Mammalian Cell Growth In Space.Microgravity Science And Technology,115-120. |
| MLA | 孙树津,et al."A Novel Counter Sheet-Flow Sandwich Cell Culture Device For Mammalian Cell Growth In Space".Microgravity Science And Technology (2008):115-120. |
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