Knowledge Management System of Institue of Mechanics, CAS
| Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry | |
Wang ZH(王战会); Jin G(靳刚) ; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China.
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| Source Publication | Journal of Immunological Methods
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| 2004 | |
| Volume | 285Issue:2Pages:237-243 |
| ISSN | 0022-1759 |
| Abstract | In the development of biosensors, the immobilization of biomolecules at interfaces played a crucial role. The feasibility of using 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify silicon surface to immobilize covalently protein for immunoassay with the biosensor based on imaging ellipsometry was investigated. The higher density and stability of human IgG layer could be obtained on the silicon surface modified with APTES and Glu than that on the silicon surface modified with dichlorodimethylsilane (DIDS). The human IgG molecules immobilized covalently on APTES-Glu surface bound more anti-IgG molecules than that on DDS surface, which indicated that the human IgG molecules could maintain higher binding capability on APTES-Glu surface. Tween 20 was able to block the undesirable adsorption on APTES-Glu surface, and also enhanced the recognition between human IgG and its antibody on both APTES-Glu and DDS surfaces. The combination of this protein covalent immobilization and the biosensor has the potential to be developed into a fast, simple immunoassay technique. |
| Subject Area | 力学 |
| DOI | 10.1016/j.jim.2003.12.002 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000220140200009 |
| WOS Keyword | SILICA SURFACES ; VISUALIZATION ; ANTIBODY ; INTERFACES ; PLASMA |
| WOS Research Area | Biochemistry & Molecular Biology ; Immunology |
| WOS Subject | Biochemical Research Methods ; Immunology |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/15439 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China. |
| Recommended Citation GB/T 7714 | Wang ZH,Jin G,Jin, G . Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry[J]. Journal of Immunological Methods,2004,285,2,:237-243. |
| APA | 王战会,靳刚,&Jin, G .(2004).Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry.Journal of Immunological Methods,285(2),237-243. |
| MLA | 王战会,et al."Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry".Journal of Immunological Methods 285.2(2004):237-243. |
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